Abstract: A theoretical calculation model based on the ergonomic evaluation of team performance was proposed in order to carry out the ergonomic evaluation of the layout design schemes of the action station in a multitasking operational room. This model was constructed in order to calculate and compare the theoretical value of team performance in multiple layout schemes by considering such substantial influential factors as frequency of communication, distance, angle, importance, human cognitive characteristics and so on. An experiment was finally conducted to verify the proposed model under the criteria of completion time and accuracy rating. As illustrated by the experiment results, the proposed approach is conductive to the prediction and ergonomic evaluation of the layout design schemes of the action station during early design stages, and provides a new theoretical method for the ergonomic evaluation, selection and optimization design of layout design schemes.